作者查詢 / seal0413
作者 seal0413 在 PTT [ Biotech ] 看板的留言(推文), 共45則
限定看板:Biotech
1F推:Good Lab!!! Professor is a beauty!!!07/24 14:20
1F→:Check your reagent!!!07/22 16:52
6F→:I mean that you can check reagent to eliminate07/22 16:27
7F→:trouble matter.07/22 16:28
8F→:I think PCR is cheaper than real time PCR07/22 16:33
9F→:PCR is a possible way to check your actin in gel07/22 16:38
10F→:easily. I think 60bp is ok for PCR to show in gel!07/22 16:41
11F→:Maybe you can see the actin expression in PCR07/22 16:44
12F→:But it's no expression in real-time PCR07/22 16:45
13F→:Good luck!!! Keep going!!!07/22 16:47
1F推:If your buffer has problem, your marker won't be07/22 05:45
2F→:showed clear.07/22 05:49
3F→:Maybe you should check your PCR reagent07/22 06:11
4F→:If PCR is ok, you will try real-time PCR!!!07/22 06:13
26F→:you should have positive control in your experiment07/21 21:06
27F→:Check your primers!!!07/21 21:08
9F→:How long did you treat your DNA by klenow fragment??07/14 19:21
7F推:可能是taq enzyme...建議應該是要你做positive control06/27 18:20
7F推:淡黃色+206/27 10:24
4F推:我有gate...fsc-ssc gate fl2a-fl2h06/27 10:31
5F→:triton不一定要加吧...不過我會固定至少overnight06/27 10:32
6F→:我的gate不管cell cycle和看subG1都有...只是fl2a-fl2h06/27 10:34
7F→:圈的細胞群會有差06/27 10:35
1F推:之前已經有paper確定敗血症引發的apoptosis是因caspase306/27 11:24
2F→:嗎?何不先確定真的有apoptosis的現象差異再看caspase306/27 11:25
3F推:何不試試看其他的 ex:DNA fragmentation06/27 11:29
4F→:要不做切片染annexin 也很 ok06/27 11:31
7F推:apoptosis有caspase dependent or independent06/27 23:55
8F→:因為你是組織的wb 所以建議你可以放positive control06/27 23:57
9F→:若真的是caspase3 或許是因為你組織蛋白太濃而有這樣的06/27 23:59
10F→:結果~或許減少蛋白量就有差異了~祝你口試順利囉06/28 00:00