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[方法] PVDF 廠牌

[ Biotech ]8 留言, 推噓總分: +4

作者: spirithorse - 發表於 2013/03/26 16:32(11年前)

3^F推kirby0415:可以請別間廠商提供試用品03/26 22:56

[求救] Co-IP

[ Biotech ]25 留言, 推噓總分: +5

作者: b715306 - 發表於 2013/03/25 12:45(11年前)

12^F→kirby0415:室溫....03/26 22:57

[求救] IHC染核內protein的方法

[ Biotech ]4 留言, 推噓總分: +3

作者: cmublue - 發表於 2013/02/21 21:25(11年前)

3^F推kirby0415:我染核沒在用triton耶試試citric acid pH8或10 或boric02/22 00:30

4^F→kirby0415:acid+EDTA pH802/22 00:31

[求救] Far-western blot

[ Biotech ]21 留言, 推噓總分: +7

作者: nhxcyge - 發表於 2012/05/21 13:24(12年前)

18^F推kirby0415:transfer後用guanidine-HCl denature後,TTBS renature,05/23 14:59

19^F→kirby0415:milk blocking, 加入prey protein, 之後再加入可以認05/23 15:00

20^F→kirby0415:prey protein的Ab~我真心懷疑原po跟我當初同間lab.....05/23 15:01

[求救]無法用Western Blot跑出有活性的Caspase-3

[ Biotech ]8 留言, 推噓總分: +3

作者: keroromoa - 發表於 2012/04/11 18:23(12年前)

2^F推kirby0415:有確定treat的dose會造成細胞的apoptosis嗎?04/11 22:06

3^F→kirby0415:要不要另外多壓一個PARP試試?04/11 22:06

4^F→kirby0415:有的時候實驗換人做,condition還是要再抓一下...04/11 22:08

[求救] 測jurkat產IL-2 cytokine

[ Biotech ]7 留言, 推噓總分: +2

作者: bearyuan - 發表於 2012/02/22 21:40(12年前)

1^F推kirby0415:condition medium中的IL-2含量大概多少?需要濃縮嗎?03/02 18:06

2^F→kirby0415:cytokine量太少有時要用Amicon濃縮一下才測的到03/02 18:06

3^F→kirby0415:如果含量真的很少有一些ELISA Kit號稱非常sensitive03/02 18:07

4^F→kirby0415:也許也可以試試看~03/02 18:07

5^F→kirby0415:總之要先確定你沒加藥的Jurkat 一定要分泌IL-203/02 18:08

[求救] PCR做不出來(已爬文)

[ Biotech ]25 留言, 推噓總分: +12

作者: hshinyi - 發表於 2010/02/22 23:54(14年前)

2^F推kirby0415:DNA 1ul的濃度是多少?02/23 11:51

[求救] 請問誰有MCF-7細胞 (台中)

[ Biotech ]3 留言, 推噓總分: +1

作者: mito0222 - 發表於 2009/11/04 21:11(14年前)

1^F推kirby0415:試試non heat inactivate serum,我記得之前Lab買來的11/05 20:48

2^F→kirby0415:MCF7好像是用沒煮過的serum喔11/05 20:48

[求救] cDNA量很低

[ Biotech ]15 留言, 推噓總分: +7

作者: icome - 發表於 2009/10/16 15:34(14年前)

3^F推kirby0415:第4步可以-20或-80 overnight10/16 20:49

[求救] conditional medium的protein extraction

[ Biotech ]1 留言, 推噓總分: +1

作者: honey823 - 發表於 2009/10/09 14:51(14年前)

1^F推kirby0415:試試看用serum free做之後TCA沉澱10/10 20:56

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