作者查詢 / kirby0415
作者 kirby0415 在 PTT [ Biotech ] 看板的留言(推文), 共88則
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15F推:我也出現過很多次...我們老師是說我製膠的玻片不乾淨02/18 20:30
16F→:我自己也不曉得為什麼會這樣,個人認為很隨機...>"<02/18 20:30
17F推:可能製膠過程出問題吧?!02/18 20:33
1F推:應該是擔心freeze and thaw太多次不好吧~我們家很多Ab也02/09 22:25
2F→:會加glycerol後凍-8002/09 22:27
1F推:我transfer都固定電流300mA~低分子量約1~1.5hrs02/08 15:50
1F→:要是我會壓在同一片膠耶...02/08 15:51
1F推:在室溫一抗通常2~3hrs即可,且室溫probe background會高02/07 22:09
2F→:我通常一抗都4度O/N或室溫hrs耶!建議做一個mouse IgG02/07 22:11
3F→:為一抗的control,這樣比較可以確定你要看的band02/07 22:11
4F→:我第二行漏打了...囧rz~是室溫2hrs02/07 22:12
5F推:短路嗎?你用哪牌的跑膠器?你只跑一片嗎?背面那面沒膠的12/24 22:08
6F→:底下電線是不是有浸到running buffer?有的話會短路喔!12/24 22:09
7F→:背面的電線不要浸到buffer12/24 22:09
8F→:壓片糊糊的...你底片有整個"用力"壓在membrane上嗎?12/24 22:11
9F→:我之前就只有"蓋"上去 就糊糊的 XD12/24 22:11
13F推:是阿是阿~~12/26 21:08
19F→:我們家買的很臭....XD12/24 22:12
4F推:你要看的protein大小多大?我通常看100kD以下的都是用12/22 22:19
5F→:300mA 1.5hrs,以上的話就2hrs!有可能是電流太強過頭了12/22 22:20
6F→:怕是沒transfer完全,可以把transfer完的gel去染12/22 22:21
7F→:coomassie!!我的marker都load 4ul,很ok喔!!12/22 22:22
1F推:推alexa488 不太quench~PE蠻亮但很容易quench11/17 23:39
2F→:PECy5用顯微鏡看不太好,但跑FACS不錯,alexa不能跑facs11/17 23:40
3F→:但顯微鏡看很強!!11/17 23:41
4F→:其實我覺得最主要看你要看的東西是甚麼還有你的一抗特不11/17 23:41
5F→:特異...11/17 23:41
6F推:我們家除了alexa488之外 其它好像都是s.c.的吧...11/17 23:44
7F→:S.C.的二抗在我們家大概都是1:150~600不等 但alexa48811/17 23:44
8F→:用來看我們家要看的東西的時候只要1:200011/17 23:45
9F→:但忘記是哪家的了 囧11/17 23:45
26F推:我一二抗都是1%BSA,一抗前先goat IgG block11/19 22:15
1F→:1.5mA?11/12 22:31