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作者 celestial318 在 PTT [ Biotech ] 看板的留言(推文), 共85則

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[求救] 24小時和48小時沒有在現性

[ Biotech ]25 留言, 推噓總分: +8

作者: U938147 - 發表於 2013/10/20 19:22(10年前)

23^F推celestial318:請其他人跑同一組sample看看10/22 15:12

[求救] Cell transfection efficiency

[ Biotech ]4 留言, 推噓總分: +2

作者: poulnew - 發表於 2011/07/25 23:03(13年前)

3^F→celestial318:四種一起送或分開送好像有差07/26 13:16

Re: [討論] 磷酸化與非磷酸化蛋白

[ Biotech ]9 留言, 推噓總分: +4

作者: FENOR - 發表於 2011/04/24 14:56(13年前)

6^F→celestial318:之前壓phospho都要用BSA blocking...04/25 00:26

[求救] p38不見了

[ Biotech ]8 留言, 推噓總分: +2

作者: anbiarsy - 發表於 2011/04/20 20:37(13年前)

5^F推celestial318:結果二抗用錯(誤04/20 22:52

[求救] western總是跑出兩條band

[ Biotech ]5 留言, 推噓總分: +2

作者: ververia - 發表於 2011/04/15 16:28(13年前)

1^F推celestial318:我也覺得是二抗你可以再把兩條band確認看看開一點04/15 17:21

2^F→celestial318:跑開一點確認= =04/15 17:21

[方法] 關於Caspase3和PARP的Western以及細胞週期相關問題

[ Biotech ]25 留言, 推噓總分: +6

作者: FUJI0904 - 發表於 2011/04/11 12:09(13年前)

1^F→celestial318:caspase-3我記得不難壓欸...雖然很久以前的事= =04/11 13:45

10^F推celestial318:嗯...以前用IMGENEX的casp-3 pro跟cleaved都有04/11 21:56

18^F推celestial318:loading多一點或抗體濃一點試試看XD04/12 13:10

[求救] p53磷酸化的結果不一致

[ Biotech ]16 留言, 推噓總分: +7

作者: penguinanny - 發表於 2011/04/01 00:37(13年前)

4^F推celestial318:下次請學姐看你做...盡量步驟一致04/01 21:23

9^F推celestial318:確認兩株細胞內protein的basal level04/01 22:33

Re: [問題] 養細胞&養細菌的注意事項

[ Biotech ]5 留言, 推噓總分: +3

作者: alicialai - 發表於 2011/03/17 05:28(13年前)

4^F推celestial318:養細胞真的是件很緊張的事XD03/17 18:24

[求救] 跑western時我的marker以及protein會連成山峰狀

[ Biotech ]8 留言, 推噓總分: +5

作者: tinaminikimo - 發表於 2011/02/12 18:42(13年前)

7^F推celestial318:感覺是buffer問題..02/13 11:44

[求救]DNA polyacrylamide 膠跑出來有重疊的影像

[ Biotech ]6 留言, 推噓總分: +2

作者: cji4cjp6 - 發表於 2010/12/02 11:05(13年前)

4^F→celestial318:我們TEMED也是放4度12/02 21:58

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