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作者 theowing 在 PTT [ Biotech ] 看板的留言(推文), 共9則

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[求救] 跑western幾個步驟上的問題

[ Biotech ]8 留言, 推噓總分: +1

作者: emilyliu - 發表於 2012/07/17 21:46(13年前)

6^F→theowing:有些protocol會寫室溫一小時或4C過夜07/20 04:44

7^F→theowing:如果跟抗體很熟可以自由組合若要確保好看的結果07/20 04:44

8^F→theowing:就一抗二抗都過夜在冰箱搖07/20 04:45

[求救] SDS PAGE (western blot) 6x dye

[ Biotech ]12 留言, 推噓總分: +4

作者: pinkkitty8 - 發表於 2012/06/16 23:25(13年前)

12^F→theowing:我們實驗室就用6XS的沈澱不是問題要用前加溫一下下就OK07/20 04:46

[求救] IF免疫染色的E-cadherin怎麼染才好??

[ Biotech ]8 留言, 推噓總分: +2

作者: Blibra1002 - 發表於 2012/05/14 15:32(13年前)

5^F→theowing:我們實驗室都稱Santa Cruz為Santa Crap...07/20 04:47

6^F→theowing:以前試過HUVEC 用Alexis染VE-Cadherin IF蠻漂亮的07/20 04:49

7^F→theowing:2% PFA固定15分鐘07/20 04:49

[求救] 請問大家做ELISA的經驗..

[ Biotech ]16 留言, 推噓總分: +4

作者: aspiren - 發表於 2009/12/07 00:36(16年前)

11^F推theowing:吸兩段排一段比較不容易有泡泡加上貼壁可以很乾淨12/07 15:24

Re: 問題 pipetman的使用

[ Biotech ]7 留言, 推噓總分: +3

作者: gunking - 發表於 2008/07/12 00:01(17年前)

5^F推theowing:還有flow裡的pipetboy 不知道是不是取名時的創意07/12 11:24

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