作者查詢 / lunarblue

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作者 lunarblue 在 PTT [ Biotech ] 看板的留言(推文), 共14則

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[求救] 改變凝膠速度及APS、TEMED用量問題

[ Biotech ]59 留言, 推噓總分: +15

作者: lyonalaya - 發表於 2017/05/11 16:40(7年前)

44^F推lunarblue: 是該先搞懂原理，但是話可以好好說，人家是上來"求救"05/18 19:41

45^F→lunarblue: 的，自己有這方面知識的話，可以提供他方向請他再去查05/18 19:43

46^F→lunarblue: 即可05/18 19:43

[求救] compound分裝後失效了

[ Biotech ]16 留言, 推噓總分: +3

作者: knowledge265 - 發表於 2016/12/21 12:22(7年前)

6^F推lunarblue: 基本上溶液態的試樣可能因為小尺度的aggregation或是與12/21 19:31

7^F→lunarblue: 溶劑作用而使其有效濃度降低，特別的是DMSO也具有一定12/21 19:32

8^F→lunarblue: 化學活性，因此即使分管保存，仍會有壞掉的可能。此外12/21 19:33

9^F→lunarblue: 溶劑中所含有的氧氣亦有可能造成氧化性的化學轉換12/21 19:33

10^F→lunarblue: 當然這種現象不是一定會發生，畢竟DMSO的確是個很好使12/21 19:34

11^F→lunarblue: 用的溶劑。解決方法其一為上面推文所述，重新以powder12/21 19:35

12^F→lunarblue: 配置stock soln，其二為若化合物珍貴不易取得，則可建12/21 19:35

13^F→lunarblue: 議就現有溶液中重新純化取得純化合物。12/21 19:36

14^F→lunarblue: 若發現此現象，爾後的實驗規劃需注意調整實驗日程，儘12/21 19:38

15^F→lunarblue: 量新鮮配置試樣12/21 19:38

16^F→lunarblue: 東西不是冰起來就不會壞的～12/21 19:38

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