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[求救] 使用Hoefer電泳槽時該怎麼做才不會弄破陶瓷片

[ Biotech ]27 留言, 推噓總分: +13

作者: yoko0920 - 發表於 2008/11/12 01:27(15年前)

20^F推kirby0415:膠不要太乾才去打開,用點水RINSE!我都是用specar去撬開11/12 22:28

21^F→kirby0415:spacer不要只去撬一個小三角形,要整個平行的撬開11/12 22:29

22^F→kirby0415:就是把SPACER往下拉出來一點點,整個平行撬開11/12 22:30

[求救] pristane打進老鼠後....

[ Biotech ]4 留言, 推噓總分: +2

作者: kirby0415 - 發表於 2008/11/10 21:26(15年前)

2^F→kirby0415:是喔!!那請問有說要隔多久才能再打嗎?謝謝~11/10 21:31

4^F→kirby0415:恩!!謝謝囉!! Orz11/10 22:43

[求救] SDS-PAGE 玻璃價格

[ Biotech ]31 留言, 推噓總分: +22

作者: skybreeze - 發表於 2008/11/05 13:54(15年前)

15^F推kirby0415:我們家應該是用普通玻璃吧!跑起來很OK!有需要可以11/05 22:43

16^F→kirby0415:幫你問價錢!(因為我們家一定是買便宜貨 XD)好像幾十元吧11/05 22:43

Re: [求救] 關於PMSF的配法

[ Biotech ]2 留言, 推噓總分: 0

作者: sharkcell - 發表於 2008/11/05 01:39(15年前)

2^F→kirby0415:我們家是用acetone耶11/05 22:45

[求救] 請問freeze solution比例

[ Biotech ]5 留言, 推噓總分: +2

作者: wej369 - 發表於 2008/10/19 11:28(15年前)

2^F推kirby0415:推樓上~但解凍後存活率不好也牽涉到凍跟解凍時的方式...10/19 12:12

[求救] DNA電泳膠

[ Biotech ]14 留言, 推噓總分: +6

作者: youngssq - 發表於 2008/10/17 14:04(15年前)

11^F推kirby0415:要看DNA多大?你用的是多少%的膠?還有你有load marker嗎?10/18 21:59

[求救] IP技術

[ Biotech ]3 留言, 推噓總分: +2

作者: qqqpig - 發表於 2008/05/03 00:25(16年前)

1^F推kirby0415:洗beads時,soln不要用suction吸掉,用pipette去吸05/04 23:37

2^F推kirby0415:或是一開始取beads時,可以用麥克筆在tube上面,beads最05/04 23:40

3^F→kirby0415:上面畫線,避免在洗的時候吸到那條線以下...05/04 23:41

[求救] transfer出問題！！

[ Biotech ]14 留言, 推噓總分: +8

作者: lblackpig - 發表於 2008/04/04 22:49(16年前)

3^F推kirby0415:泡transfer buffer+104/05 08:52

[求救] 有關HEK293 transfect的實驗.....

[ Biotech ]11 留言, 推噓總分: +8

作者: JinMinHo0626 - 發表於 2007/11/15 17:39(16年前)

6^F推kirby0415:改用ad293如何?我覺得ad比Hek不容易飄耶....11/15 20:29

[問題] RNA電泳相關問題

[ Biotech ]5 留言, 推噓總分: +5

作者: chvkimo - 發表於 2007/09/16 23:48(17年前)

4^F推kirby0415:MOPS+109/17 18:58

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