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作者 roy047 在 PTT [ Biotech ] 看板的留言(推文), 共230則

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[求救] THP-1細胞不貼

[ Biotech ]11 留言, 推噓總分: +1

作者: wolfling22 - 發表於 2014/07/01 10:53(10年前)

4^F→roy047:incubator CO2那些應該都正常吧？07/01 14:39

[求救] 第二代 Lenti packing system 質體純化

[ Biotech ]28 留言, 推噓總分: +5

作者: silverberry - 發表於 2014/06/04 05:07(10年前)

2^F→roy047:該midi抽其他plasmid正常嗎？06/04 08:07

[求救] western repeat不出來

[ Biotech ]4 留言, 推噓總分: +1

作者: aassdzx - 發表於 2014/05/27 17:46(10年前)

2^F→roy047:可能很容易degrade, 新鮮製備protein看看05/27 18:33

3^F→roy047:還沒加SB煮的放-80也撐不久建議加SB煮完保存, 放-20較穩05/27 18:34

[求救] western 跑完膠玻璃破掉

[ Biotech ]9 留言, 推噓總分: +2

作者: bear2926 - 發表於 2014/05/07 10:05(10年前)

4^F→roy047:如果要買Biorad副廠玻璃我們是跟百聯生技買05/07 12:35

[求救] SDS-page 底下一條線分不開

[ Biotech ]10 留言, 推噓總分: +4

作者: windcloud27 - 發表於 2014/05/06 22:06(10年前)

3^F→roy047:白金絲看有沒有鏽掉換tank看看05/06 23:33

6^F→roy047:如果做膠時沒凝好上下膠交界太多水的話跑到那邊會漫開05/07 07:04

[討論] 國考題目求解

[ Biotech ]8 留言, 推噓總分: +2

作者: caesar0926 - 發表於 2014/04/20 17:15(10年前)

8^F→roy047:類似序列: 朝 degenerate PCR 方向來寫?04/21 20:53

[討論] 如何判斷電泳的band為RNA 或DNA?

[ Biotech ]7 留言, 推噓總分: +1

作者: goodday06 - 發表於 2014/04/10 21:21(10年前)

1^F→roy047:加DNase?04/10 22:07

[求救] Western後band定量分析

[ Biotech ]2 留言, 推噓總分: +1

作者: neverleave45 - 發表於 2014/04/08 14:44(10年前)

1^F→roy047:強度peak圖形對調而已吧還好04/08 16:58

[求救] 切位是正確的但是切不下來

[ Biotech ]5 留言, 推噓總分: +1

作者: bluesnow1122 - 發表於 2014/03/27 11:24(10年前)

5^F→roy047:樓上正解~ 沒dam就不會甲基化了 plasmid seq不受影響03/27 13:41

[求救] 細胞汙染

[ Biotech ]14 留言, 推噓總分: +4

作者: mg880613 - 發表於 2014/03/17 11:58(10年前)

1^F→roy047:細胞凍時就汙染03/17 12:01

2^F→roy047:看起來像是常見細菌汙染03/17 12:02

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