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作者 turtle24 在 PTT [ Biotech ] 看板的留言(推文), 共621則

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[求救] protein sample煮過頭

[ Biotech ]21 留言, 推噓總分: +7

作者: migration - 發表於 2022/03/22 16:18(3年前)

1^F→turtle24: 定量完有剩就花15分鐘重配重煮啊03/22 17:43

[閒聊] 外用pipette和無菌操作共用？

[ Biotech ]42 留言, 推噓總分: +5

作者: steve42125 - 發表於 2022/03/02 02:26(3年前)

5^F→turtle24: 操作台跟廠商議價，多的錢買pipetman03/02 13:16

從美國運送老鼠

[ Biotech ]8 留言, 推噓總分: +4

作者: jiori1204 - 發表於 2021/11/18 22:54(4年前)

2^F→turtle24: 看這個老鼠jax有沒有寄存，會比較輕鬆11/19 10:47

3^F→turtle24: 跟jax買老鼠，文件費～200，運費看籠數也幾百美金11/19 10:49

4^F→turtle24: 也可以問問樂斯科有沒有代辦進口的服務，花比較多錢但11/19 10:52

5^F→turtle24: 是手續比較輕鬆……11/19 10:52

[求救] Hepa 1-6 cell line 小鼠肝癌細胞株

[ Biotech ]2 留言, 推噓總分: 0

作者: hachibuya - 發表於 2021/09/09 22:06(4年前)

2^F→turtle24: 這個買得到不用這麼搞剛吧09/11 22:26

[求救] 實驗小鼠購買

[ Biotech ]26 留言, 推噓總分: +5

作者: Nroh - 發表於 2021/01/28 15:15(4年前)

1^F→turtle24: 進口貴很多耶，樂斯科不是有嗎？01/29 01:14

[求救] PBMCs上flow但抗體染不上去

[ Biotech ]35 留言, 推噓總分: +5

作者: isorange - 發表於 2020/11/08 21:33(5年前)

18^F→turtle24: 兩個人相同材料各做一次，先排除人的問題11/09 11:39

[求救] 被切EtBr膠的刀子刺到……

[ Biotech ]18 留言, 推噓總分: +7

作者: wengbilly - 發表於 2020/09/23 14:54(5年前)

7^F→turtle24: 不用打破傷風嗎？新刀舊刀？09/24 01:47

[求救]T4 ligation

[ Biotech ]14 留言, 推噓總分: +3

作者: janewu945 - 發表於 2020/09/14 23:02(5年前)

6^F推turtle24: 挑完菌再切吧，然後建議選別的切位測試09/15 12:43

7^F→turtle24: ligation的產物又切同樣酵素不就insert跟vector混合物09/15 12:44

8^F→turtle24: 嗎09/15 12:44

[求救] 1-methyl-L-tryptophan（1-MT)溶解方法

[ Biotech ]12 留言, 推噓總分: +2

作者: catmua0507 - 發表於 2020/07/20 21:22(5年前)

11^F→turtle24: 建議把重量跟溶解體積打一下，說不定算錯07/22 09:41

12^F→turtle24: 如果一開始就算錯十倍百倍，可能比芝麻糊還稠07/22 09:43

[求救] western 上膠會縮

[ Biotech ]32 留言, 推噓總分: +7

作者: Smallforward - 發表於 2020/07/03 00:20(5年前)

17^F→turtle24: 玻璃如果是自己去玻璃行切的，長尾夾可能會夾斷玻璃07/04 00:18

18^F→turtle24: （受力不均的情形下）07/04 00:19

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