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作者 roy047 在 PTT [ Biotech ] 看板的留言(推文), 共230則

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[求救] western blot 拍到mark!!

[ Biotech ]9 留言, 推噓總分: +3

作者: vipeyu - 發表於 2014/11/27 17:56(9年前)

6^F→roy047: 有些抗體會認到marker沒錯11/28 06:55

[求救] 羊可以輸其他羊的血嗎?

[ Biotech ]14 留言, 推噓總分: +6

作者: g318 - 發表於 2014/10/19 19:05(9年前)

6^F→roy047: 同意三樓10/20 08:36

[討論] 快速做出cloning送定序的方法

[ Biotech ]5 留言, 推噓總分: +3

作者: gotstarch - 發表於 2014/10/14 13:39(9年前)

2^F→roy047: 同一樓, 不過開頭會定不太到, 可P長一些10/14 15:55

[討論] 有關動物疫苗問題~

[ Biotech ]5 留言, 推噓總分: +1

作者: satasumi - 發表於 2014/09/29 01:22(9年前)

1^F→roy047: 沒有寫是哪種pathogen...09/29 10:09

[求救] SDS-PAGE跑膠電泳槽一直發燙冒泡

[ Biotech ]9 留言, 推噓總分: +3

作者: albee1992822 - 發表於 2014/09/23 16:27(9年前)

3^F→roy047: 看一下電極線有沒有生鏽或卡東西09/23 17:15

[求救] 合成兩條單股DNA再Annealing

[ Biotech ]11 留言, 推噓總分: +4

作者: Dusha - 發表於 2014/09/17 17:53(9年前)

1^F→roy047: primer應該可以設計成annealing後直接有overhang, 不用RE09/17 18:36

[問題] western blot 跑膠疑問

[ Biotech ]12 留言, 推噓總分: +5

作者: hahabighan - 發表於 2014/08/11 15:22(9年前)

2^F→roy047: 2.相反+1, 到resolving下膠時才能加速...08/11 16:13

3^F→roy047: 1.的話是SDS會附在蛋白上08/11 16:14

[求救] ELISA kit 保存期限

[ Biotech ]3 留言, 推噓總分: 0

作者: gozird - 發表於 2014/07/24 10:07(10年前)

2^F→roy047:做做看standard curve還拉不拉得出來07/24 15:21

[求救] 發酵培養基的顏色

[ Biotech ]3 留言, 推噓總分: +1

作者: CCDMer - 發表於 2014/07/21 11:39(10年前)

2^F→roy047:用0.22或0.45 um filter過濾07/21 16:00

[求救] Lentivirus titer 測定

[ Biotech ]22 留言, 推噓總分: +3

作者: silverberry - 發表於 2014/07/19 15:38(10年前)

5^F推roy047:類似二樓取少量virus感染少量cell看GFP，也有lab是跑qPCR07/20 06:21

14^F→roy047:如果原本沒GFP加了GFP表現量會不一樣所以也不需要硬加07/21 07:10

15^F→roy047:可以改用別的方法測除非以後實驗全都換成有GFP的07/21 07:11

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