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作者 roy047 在 PTT [ Biotech ] 看板的留言(推文), 共230則

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[求救] PCR product 定序

[ Biotech ]15 留言, 推噓總分: +3

作者: shinchenboy - 發表於 2015/04/13 16:22(9年前)

9^F→roy047: 可反方向定回來即可知道最開頭序列04/13 18:54

[求救] 跑SDS-PAGE時上膠的WELL會漏

[ Biotech ]3 留言, 推噓總分: +1

作者: brutalsky - 發表於 2015/04/01 12:30(9年前)

3^F→roy047: 注上膠前中間水沒倒乾淨or上膠凝固不均勻？04/01 13:39

[求救] 最近細胞狀況不好

[ Biotech ]9 留言, 推噓總分: +1

作者: peterjfs - 發表於 2015/03/23 22:18(9年前)

1^F→roy047: incubator是好的嗎?@@03/23 22:44

[求救] 96well孔盤產生的氣泡

[ Biotech ]13 留言, 推噓總分: +6

作者: Kraft - 發表於 2015/03/13 20:22(9年前)

3^F→roy047: 樓上正解熱針頭一戳泡泡就會破03/13 22:40

[期刊] CEACAM1和TIM3報導(P幣酬謝)

[ Biotech ]1 留言, 推噓總分: 0

作者: vancent - 發表於 2015/02/25 11:27(9年前)

1^F→roy047: 已寄出, P幣就不用了QQ02/25 14:38

Western band定量出的ratio不穩定（再現性差）

[ Biotech ]31 留言, 推噓總分: +8

作者: snow122 - 發表於 2015/02/02 21:59(9年前)

1^F→roy047: loading前盡量vortex, pipetting均勻02/02 22:20

[求救] [生化] 一題台大生化實驗題組

[ Biotech ]42 留言, 推噓總分: +13

作者: dcpick2001 - 發表於 2015/02/01 21:40(9年前)

5^F→roy047: 應該lane"4"才是double digestion, 切出1.5kb就是cDNA02/02 07:26

6^F→roy047: 錯字不少02/02 07:26

[討論] 點火器瓦斯充氣要怎麼充才對？

[ Biotech ]5 留言, 推噓總分: +2

作者: LIAR - 發表於 2015/01/11 20:31(9年前)

1^F→roy047: 在hood內充?01/11 21:51

Re: [方法]請問WB的band要怎樣壓片讓他看起來比較圓?

[ Biotech ]39 留言, 推噓總分: +8

作者: t123401221 - 發表於 2015/01/07 10:26(9年前)

26^F→roy047: 定量, loading膠前必先vortex, spin down才不會吸不均勻01/07 13:45

27^F→roy047: 或定錯量01/07 13:45

33^F→roy047: sample先vortex確保混勻之後spin down 吸取01/07 15:56

34^F→roy047: 因vortex完有些會在管壁上01/07 15:56

35^F→roy047: 因為如果不均勻, 吸的protein量就時多時少了, 會造成誤差01/07 15:57

[方法]請問WB的band要怎樣壓片讓他看起來比較圓?

[ Biotech ]7 留言, 推噓總分: +2

作者: t123401221 - 發表於 2015/01/06 18:00(9年前)

5^F→roy047: GAPDH一抗1:1000....01/06 18:44

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