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作者 Rinehot 在 PTT [ Biotech ] 看板的留言(推文), 共10則
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[求救] site-directed mutagenesis做不出來
[ Biotech ]18 留言, 推噓總分: +3
作者: even2300471 - 發表於 2011/03/31 15:28(13年前)
1F→Rinehot:你PCR完有沒有跑膠? 確認一下有沒有P到東西吧03/31 16:01
[求救] His-tag protein 純化問題
[ Biotech ]29 留言, 推噓總分: +6
作者: relaxsleep - 發表於 2010/07/16 08:12(14年前)
7F→Rinehot:有用antiHis確認過有沒有表現嗎?07/16 16:02
10F→Rinehot:你染色的方式是?07/16 18:55
11F→Rinehot:有可能是protein上的his跟Ni結合力太低07/16 18:56
12F→Rinehot:可以試試看加入少量的detergent glycerol07/16 18:58
[求救] 請問his tag的純化方法
[ Biotech ]7 留言, 推噓總分: +5
作者: championelmo - 發表於 2010/07/09 18:12(14年前)
7F→Rinehot:你的buffer條件還有蛋白性質也很重要07/12 15:45
[求救] 透析出現沉澱
[ Biotech ]7 留言, 推噓總分: +3
作者: bolly - 發表於 2010/06/11 00:14(14年前)
4F→Rinehot:慢慢降低+106/11 11:29
[求救]transfection後蛋白質表現不出來
[ Biotech ]8 留言, 推噓總分: +3
作者: doskoi - 發表於 2009/10/15 05:17(14年前)
3F→Rinehot:建議測試一下antibody 曾有壞掉然後以為沒表現的經驗過10/15 13:02
[求救] CD peak判讀
[ Biotech ]2 留言, 推噓總分: +2
作者: power384 - 發表於 2009/10/14 00:48(14年前)
2F推Rinehot:你要看看的樣品中的成分 有時候Buffer的訊號會有影響10/18 00:20
[轉錄]開發基金亂花全民二億元內幕 翁啟惠、何美玥角 …
[ Biotech ]1 留言, 推噓總分: +1
作者: kaikai1112 - 發表於 2008/01/11 09:49(16年前)
6F→Rinehot:生技產業本來就是燒錢 有沒有燒出成果運氣很重要01/10 23:07
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