作者查詢 / mesenchymal
作者 mesenchymal 在 PTT [ Biotech ] 看板的留言(推文), 共259則
限定看板:Biotech
看板排序:
全部Liverpool4110Road_Running1402nCoV20191247Gossiping1159Baseball418FAPL388NBA299NY-Mets283MLB270Biotech259TypeMoon240Mo-Musume199FITNESS197studyabroad144C_Chat132movie106Palmar_Drama84Wine41cookclub35WorldCup31MuscleBeach28Celtics24NewYork21Coffee19TOEFL_iBT17Bioindustry14wearefriends11fastfood10EAseries8FuMouDiscuss8NFL8CVS7Gintama7GRE7Hunter7marvel7SuperHeroes7VISA6Lions5Suckcomic5Android4Boy-Girl4Cancer4cookcomic4HatePolitics4Hotspur4JinYong4FCBarcelona3Grizzlies3IA3junji-ITO3Chelsea2Militarylife2Nubee2Olympics_ISG2PokeMon2Realityshow2SanFrancisco2Bundesliga1clmusic1Comic1GHIBLI1God_Drop1Hawks1Hornets1Japandrama1jersey1ManCity1maruko1Master_D1Mind1mobile-game1MobileComm1Netherlands1NorthAmerica1Nuggets1NYState1Opera1Raptors1Seiya1TuTsau1Zastrology1<< 收起看板(82)
6F推: 現在有NP-40 Substitute12/29 03:35
12F推: cell line抽RNA RIN小於9都很有問題了10/13 01:39
4F推: PVT1? Myc?12/05 06:54
2F推: transfer完用Ponceau S染membrane確認第一項09/22 23:54
3F→: 搞不好就是氣泡或其它問題導致transfer不完全09/22 23:55
1F推: Tail vein injection,看轉移的話用intracardiac08/08 13:27
2F→: injection08/08 13:28
3F推: 2.大部份都24h內 3.可以試著用2-3種HF相對長的genes07/30 12:36
4F→: 當internal controls, 如(18s rRNA, beta actin...)07/30 12:37
5F推: GAPDH也有人用,但你可以先測試找出最適合的07/30 12:41
9F推: 應該說憑你的目的來決定方法07/09 22:44
6F推: ensembl 右上搜尋列打 染色體:54531-5688105/29 10:45
7F→: 搜尋就會自動列出該片段上annotated的基因了05/29 10:46
7F推: Mole(Mol) Molar(M) ?05/06 08:11
1F推: 用面積算,10cm dish約是6 well plate一個well的八倍03/27 00:32