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作者 conective 在 PTT [ Biotech ] 看板的留言(推文), 共290則
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1SKET_DANCE1SMJH-89-3091Sociology1South_NCTU1SouthPark1specialman1SSSH-16th3171SSSH_17th3141sttmountain1studyteacher1TA_AN1TaiShan1TaitungH92-11TBBT1TCSH561Teacher1teeth_salon1Tennis1TFSHS66th3121TFSHS67th3091TFSHS68th3121TFSHS68th3211TFSHS69th3071TFSHS69th3141THUIM-1st1TKU-Strategy1TKU_ACC_95A1TKU_STAT_SB1TKU_trans1toberich1TR1Trans-nthu1Transport921TRXradio1Tsao1TTSH-12th3171TTU-I90B1TTU-IM1TTU-Transfer1TurtleSoup1TuTsau1TWvoice1TYJH_92_ENG1TYSH49-1101UFO1V_ScHooL1VETsoftball1Vocal1Wallace1Windows1WorkanTravel1Wrestle1XZ25th2481YHSH96011YP95-3121Yup98-091<< 收起看板(545)
[求救] flow 對照組雙染偏右消失
[ Biotech ]16 留言, 推噓總分: +6
作者: eccman - 發表於 2016/12/21 20:22(7年前)
14Fconective: 有算細胞數嗎 你單染AV跟雙染AV背景就不同01/02 09:56
[求救] 請問"將藥物加入細胞"的英文
[ Biotech ]12 留言, 推噓總分: +6
作者: ej04cl6 - 發表於 2016/07/28 23:34(7年前)
9Fconective: dose with?07/30 22:15
[求救] 用flow測細胞存活率
[ Biotech ]17 留言, 推噓總分: +4
作者: satan317 - 發表於 2016/05/17 18:06(8年前)
1Fconective: 試試看accutase打 看起來存活率只有50% 有點太低了05/17 18:29
2Fconective: 你上普通PBS或是沒有細胞的medium也會有左下角那群嗎?05/17 18:30
[求救] Flow cytometry protocol(FITC)
[ Biotech ]35 留言, 推噓總分: +14
作者: tony51501 - 發表於 2016/03/11 18:40(8年前)
15Fconective: 一抗是ihc的 不代表可以做FLOW唷03/12 12:20
16Fconective: 看起來是沒有螢光表現03/12 12:21
30Fconective: 重點是有看到細胞 電壓值跟你細胞大小有關 沒有標準答03/14 21:52
31Fconective: 案03/14 21:52
32Fconective: 你有沒有Positive control 就是必定會染上的組別?03/14 21:54
[求救] Flow表面抗原分析
[ Biotech ]8 留言, 推噓總分: +4
作者: homeshawn - 發表於 2015/04/22 15:02(9年前)
7Fconective: Accutase 試試04/23 17:59
[閒聊] 增強實驗技能與知識
[ Biotech ]4 留言, 推噓總分: +4
作者: charliedorae - 發表於 2014/08/03 04:48(9年前)
4Fconective:CURRENT PROTOCOL08/04 00:55
[情報] lipofectamin3000價格
[ Biotech ]8 留言, 推噓總分: +4
作者: apple23418 - 發表於 2014/06/24 18:45(10年前)
2Fconective:打電話詢價會快很多....06/24 21:16
[求救] 細胞用100%EtOH固定上flow
[ Biotech ]4 留言, 推噓總分: +2
作者: MDCCLXXVI - 發表於 2014/06/12 10:31(10年前)
3Fconective:固定用70%就可以了06/13 01:12
[求救] DNA 純化問題 (DNA extraction kit)
[ Biotech ]63 留言, 推噓總分: +8
作者: ayenyayaya - 發表於 2014/03/13 14:03(10年前)
35Fconective:膠回收可能有問題 沒溶乾淨的話 純化前的量就少了03/15 03:25
36Fconective:膠回收可到70度, 回溶的水65-70度03/15 03:29
[求救] 想請教一下Annexin V/7AAD跑Flow
[ Biotech ]10 留言, 推噓總分: +4
作者: truthPP - 發表於 2013/09/21 21:37(10年前)
1Fconective:針對compensation作回覆 當你上某單染色樣品的時候09/21 22:10
2Fconective:需要扣除的就是該顏色的偵測器 換言之上AnnexinV時是09/21 22:11
3Fconective:FL3-FL2%, 上7-AAD時是 FL2-FL3% 前面為被干擾的偵測器09/21 22:11
4Fconective:後面為干擾別的偵測器的螢光(也就是你單染的螢光)09/21 22:12
5Fconective:目的是為了排除因為螢光物質發散光譜本身彼此間的干擾09/21 22:13
6Fconective:所造成的false positive 降低雙陽性比例的誤判09/21 22:13