[求救] 請教如何分離死亡與健康的懸浮式細胞?

看板Biotech作者AronYA ( )時間15年前 (2009/04/13 15:33)推噓4(4推 0噓 0→)

留言4則, 4人參與討論串1/2 (看更多)

利用 Lenti virus 做 RNAi實驗再做puromycine篩選我的細胞株是懸浮式的 puro.篩後細胞每次繼代凋亡或者不健康的細胞在離心時都會跟健康的細胞一起離下來進而影響繼代的品質想請教有沒有人知道要利用那種溶液可以將懸浮式細胞的凋亡細胞以及健康細胞分離開麻煩各位了 ~ -- You had a life before I came along. So did I. Including people we've loved, that we're still in love with. But that doesn't mean we can't love each other. -- ※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 134.208.25.92

推

daniel2806

04/13 22:46, , 1^F

04/13 22:46, 1^F

推

hughzero

04/14 09:29, , 2^F

04/14 09:29, 2^F

推

auxotroph

04/14 22:24, , 3^F

04/14 22:24, 3^F