Re: [問題]pET28a的表現效率
Hi,
Thanks for your question. To be honest with you, that was basically
my personal experience, and I have no standard answer to it. :)
I agree with the point you brought up, that it might actually take
less energy for the cellular trancription/translation machineries
to carry out a complete cycle of transcription/translation of a smaller gene.
My explanation is, there is less chance for the premature termination
of transcription/translation to ocurr since the gene is smaller.
For instance, longer genes might have more "rare codons" for the host
bacteria (bacteria have less corresponding tRNAs for these codons.
One example is the arginine codon AGA) It is believed that the rare
codons cause pausing of translation in bacteria. Therefore it takes
additional time/energy to complete a translation cycle for a longer gene.
※ 引述《Ren (wisc)》之銘言：
: ※ 引述《Janeliuu.bbs@ptt3.cc (Janeliu)》之銘言：
: : Hi,
: : I have no experience in expression of protein lower than 10kD either.
: : But I would think pET28 is an ok system, since smaller protein is
: : generally easier to express. I would give it a try. Good luck!
: Could you please explain why it's "generally easier" to express?
: Simply because it's smaller (which may require less energy)?
--
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◆ From: 68.197.145.28
※ 編輯: Janeliuu 來自: 68.197.145.28 (03/14 09:13)
※ 編輯: Janeliuu 來自: 68.197.145.28 (03/14 09:22)
※ 編輯: Janeliuu 來自: 68.197.145.28 (03/14 09:23)
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