Hi,
I have no experience in expression of protein lower than 10kD either.
But I would think pET28 is an ok system, since smaller protein is
generally easier to express. I would give it a try. Good luck!
※ 引述《statan.bbs@ptt.cc (看山 望海 吹風)》之銘言：
: 請教一下，pET28a可以大量表現10 kDa以下的蛋白質嗎？
: 因為我之前常用來表現的蛋白都在20 kDa以上。
: 這次找到的目標蛋白卻只有84個a.a.，我擔心pET28a沒辦法做出來。
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