Re: [問題] Cloning:Enzyme digestion check
※ 引述《flyseason.bbs@ptt.cc (Live.myself)》之銘言:
> ※ 引述《pneumonia.bbs@beta.life.nthu.edu.tw (JUAN)》之銘言:
> : It depends on how the "enzyme check" experiment is designed.
> The vector is pET24a+,for 6xHis-tag fusion protein
> The empty vector is about 5-6kb
> The cutting sites selected are BamHI and XhoI
> Thanks again ^^
Normally, you should be able to see the insert fragment.
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