Re: [問題] Cloning:Enzyme digestion check
※ 引述《flyseason.bbs@ptt.cc (Live.myself)》之銘言:
> I am trying to clone a 750bp fragment into a vector.
> So far I have got several candidates after transformation.
> I purified the DNA by mini-prep
> I am wondering could I see the 750bp on DNA gel after enzyme check,
> Does anyone have experiment about similar size cloning, and be successful in
> the enzyme check?
> Thank you,
It depends on how the "enzyme check" experiment is designed.
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