Re: [方法] 如何在plasmid上加Bgl II sites?
※ 引述《pneumonia.bbs@beta.life.nthu.edu.tw (JUAN)》之銘言：
: ※ 引述《oddesy.bbs@ptt.cc (馬車從天上掉下來 N)》之銘言：
: > Please tolerate that I cannot type Chinese.
: > I recently confronted a problem.
: > Now I have a plamid without Bgl II site.
: > However two Bgl II sites are required for linking to
: > a vector as a commercial kit.
: > How to make two Bgl II sites into my plasmid?
: > Thanks
: Not sure what you mean about "linking to".
: Is site-directed mutagenesis what you want?
回到家可以打中文了 呼呼
我沒有作 mutation 想在plasmid 上加Bgl II sites
我需要在我的plasmid上加上兩個Bgl II sites 才能跟廠商賣
的vector作ligation
會有這個限制是因為現成的vector上的restriction enzyme sites
是固定的其中包括Bgl II 而且kit裡有附Bgl II enzyme
拜託大家了
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