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作者 skywings28 在 PTT [ Biotech ] 看板的留言(推文), 共7則
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[求救] 純化蛋白
[ Biotech ]45 留言, 推噓總分: +7
作者: skywings28 - 發表於 2015/09/01 01:53(10年前)
5F→skywings28: 所以有可能是beads量不夠囉?還是說減少loading量下手09/01 11:32
12F→skywings28: sigma的09/02 01:09
29F→skywings28: flowthrough部分 我是in 10~15ml loading 12uL09/02 14:59
30F→skywings28: 珠子capacity為 15mg/ml gel 上面有人說實際上更低09/02 15:01
31F→skywings28: IN 指的是flowthrough整個的體積 還是煮蛋白的量?09/02 15:03
32F→skywings28: 煮蛋白我是40ulsample +10uldye09/02 15:03
39F→skywings28: 染膠的話其實主要的band 很弱 flow那個很明顯09/03 12:26
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