作者查詢 / Devilxxx
作者 Devilxxx 在 PTT [ Biotech ] 看板的留言(推文), 共33則
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18F推: 養細胞我推Hyclone08/24 16:10
2F推: 推一樓08/24 16:08
4F推: 拍照前玻片有清潔擦拭過嗎?03/21 12:59
14F推: 我之前做invasion是用80ug/mL的濃度(用medium稀釋matri06/12 00:22
15F→: gel)然後37C coating過夜,沒有吸除多餘的06/12 00:22
16F→: 查了一下當時收到的Matrigel原液濃度為9.6mg/mL 我是先06/12 00:24
17F推: 1:1稀釋成4.8mg/mL的stock全部分裝完,一次拿一管出來再06/12 00:26
18F→: 稀釋成80ug/mL的working sol做coating06/12 00:26
19F→: 一開始分裝就先算好每次只拿一管出來的量(稀釋後體積務06/12 00:27
20F→: 必超過你實際要用的總體積避免氣泡和pipetting error)06/12 00:28
21F→: 以稀釋倍率來看的話是120倍稀釋呢 所以原液濃度很重要06/12 00:28
22F推: 濃度越高那層膠會越硬,硬的話細胞吃不掉就穿不過了不是08/24 02:05
23F→: 嗎?@@08/24 02:05
16F推: 跑膠的時候有冰浴嗎?溫度升起來也有機會糊掉加上GAPDH08/24 01:59
17F→: 很下面08/24 01:59
22F推: 看來是固定沒固定好欸07/19 00:46
14F推: 30%泡一個O/N後換新的30%再泡一個O/N看看06/12 00:33
15F→: 10% formalin還是10% PFA?如果是後者的話太高 前者safe06/12 00:33
16F→: 因為10% NBF裡面的PFA也是4%06/12 00:34
10F推: formaldehyde是拿來固定細胞的,有沒有fbs好像沒啥關係03/25 20:52
11F→: ?03/25 20:52
9F推: 可是gunster那排蓋子拔起來軟趴趴的還會斷OQ02/28 20:31
12F推: 對呀連蓋的會斷 獨立蓋的很ok!03/20 23:11
9F推: 我們都送一家儀器公司磨一支15002/28 20:28