作者查詢 / Devilxxx
作者 Devilxxx 在 PTT [ Biotech ] 看板的留言(推文), 共40則
限定看板:Biotech
看板排序:
全部BigPeitou729Reptile596Aves457NTUFS-91272AquaPet196Plant111Steam98Arthropoda96BoardGame92NTUFS-9267DJ_fightman60CSI43Biotech40cat36Gossiping27Bio-Job25FTV24BeautySalon22Coffee17StarCraft16Aquarium15Exotic_Pet15NTU14TOEIC12baking11Minecraft11NTUF-CLUB11NTUFS-9011S.H.E10BigSanchung9SurvivalGame9BLEACH8Ecophilia8AiYazawa7C_Chat7NTUF-947CVS6e-shopping6EAseries6FOREST_BIO6hypermall6BERSERK5Gintama5INSECT-915NTUF-955NTUFRC-925NTUFVOLLEYBL5travian5AnimalForest4Bus4BuyTogether4Ornithology4wonfu4Bread3Expansion073Hiking3JAM_Project3MountainClub3NCUMTClub3NTUFM-913pet3Slayers3sttmountain3VET_933B97303XXX2b98902xxx2BigShiLin2Bioindustry2Biology2CODE_GEASS2marriage2NCHU-AGR042NTUFIN-912NTUIB022petwork2sinica2TRPG2WULING-B972YOLO2About_Clubs1AC_Music1AfterPhD1Agri_Service1Ai_Ching1AllTogether1AnimalGoods1b982040XX1b984060XX1B99303XXX1B99310XXX1BeautyBody1Boy-Girl1CAFENCAKE1chocolate1Claymore1CS91JAY1HandMade1hardware1home-sale1Hsinchu1HsinTien1HSNU_HIGH1job1joke1KS98-3121LCD1LinKou1LoveLive1Magic1MINGDAO1movie1nb-shopping1NCCU_SEED1NCHU-AGR021NCIS1NCTU_TALK1NDS1NTU-Archery1NTU104thLIS1ntuACCT031NTUACCTR1001NTUACCTR1011NTUACCTR1021NTUACCTR991NTUBP-R1001NTUBSE-B-1011NTUBSE-B-1021NTUBSE-B-991NTUBST981NTUDFLL-MA991NTUF-971NTUF-991NTUFIN-901NTUHistory001NTUHorti961NTUMEB981NTUOCBio1PC_Shopping1phys981Psychiatry1PT-BB1PTGS57th3031R98A41XXX1Sagittarius1Sewing1ShowOff1StupidClown1TFG00kind1TigerBlue1Transport1001TTU-US921TTU-US941VET_961VideoCard1Vietnam1WomenTalk1WOW1ZOO1<< 收起看板(158)
18F推: 養細胞我推Hyclone08/24 16:10
2F推: 推一樓08/24 16:08
23F推: sample沒處理好?05/02 18:50
4F推: 所以流速是多少,只知道RPM不知道管徑不好判斷04/23 20:20
5F→: PBS裡有加heparin嗎?個人經驗深蹲麻醉後馬上灌含hepari04/23 20:21
6F→: n04/23 20:21
7F→: 的PBS都可以很乾淨04/23 20:21
8F→: 深度麻醉Orz04/23 20:21
4F推: 拍照前玻片有清潔擦拭過嗎?03/21 12:59
18F推: biotna哪裡買呀?04/23 20:36
14F推: 我之前做invasion是用80ug/mL的濃度(用medium稀釋matri06/12 00:22
15F→: gel)然後37C coating過夜,沒有吸除多餘的06/12 00:22
16F→: 查了一下當時收到的Matrigel原液濃度為9.6mg/mL 我是先06/12 00:24
17F推: 1:1稀釋成4.8mg/mL的stock全部分裝完,一次拿一管出來再06/12 00:26
18F→: 稀釋成80ug/mL的working sol做coating06/12 00:26
19F→: 一開始分裝就先算好每次只拿一管出來的量(稀釋後體積務06/12 00:27
20F→: 必超過你實際要用的總體積避免氣泡和pipetting error)06/12 00:28
21F→: 以稀釋倍率來看的話是120倍稀釋呢 所以原液濃度很重要06/12 00:28
22F推: 濃度越高那層膠會越硬,硬的話細胞吃不掉就穿不過了不是08/24 02:05
23F→: 嗎?@@08/24 02:05
16F推: 跑膠的時候有冰浴嗎?溫度升起來也有機會糊掉加上GAPDH08/24 01:59
17F→: 很下面08/24 01:59
22F推: 看來是固定沒固定好欸07/19 00:46
14F推: 30%泡一個O/N後換新的30%再泡一個O/N看看06/12 00:33
15F→: 10% formalin還是10% PFA?如果是後者的話太高 前者safe06/12 00:33
16F→: 因為10% NBF裡面的PFA也是4%06/12 00:34