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全部Gossiping258542HatePolitics54621CSMU-LS89522PublicIssue318Tech_Job112Salary109Biotech104joke86Teacher77L_TalkandCha71IA68home-sale63AfterPhD59Bioindustry44Road_Running44DSLR37Military35Boy-Girl34Gov_owned34MdnCNhistory33NTU32StupidClown32Tainan31WomenTalk29Kaohsiung27Keelung27PingTung25PublicServan24TaichungBun24GossipPicket21Militarylife21car19historia19I-Lan19Coffee18Warfare18Daan16consumer15Stock15biker14Ecophilia13TPC_Police13Wanhua13WOW13Biology12Anti-ramp11CGI-Game11CVS11FLAT_CLUB11L_SecretGard11model11HateP_Picket10hypermall10movie10nCoV201910specialman10bicycle9ChangHua9Clarify9Employee9Examination9NextTV9TW-history9Brand8CareerPlan8Hate8LAW8photo8Violation8Magic7Marginalman7Master_D7NTUmed947Chiayi6gardener6KOTDFansClub6LTK6Mancare6Old-Games6PublicBike6SuperBike6Wego6Wine6YOLO6dog5Jay5MuscleBeach5PH-sea5rent-exp5Starbucks5studyabroad5TAKMING5TigerBlue5Tour-Manager5wonfu5Facebook4Feminism4GreenParty4kochikame4KS93-3134KTV4Little-Games4NCCU4NCKU4NTCUST4NTUEE_Lab5544SCU_BM_VB4sex4TFSHS64th3164toberich4About_Life3ask3Buddhism3CATCH3DPP3EarthScience3Education3FJU-ACC90a3Food_Safety3FSHS-95-3083gallantry3GUNDAM3KS95-3183KS96-3183LifeSci_983love-vegetal3LSSH3Miaoli3Navigation3nb-shopping3NIUCE96a3NYUST3Olympics_ISG3Paul_59-1T3PokemonGO3Post3S.H.E3Sony-style3Taipei3Taoyuan3TWproducts3Viator3Aviation2CareerLady2CFantasy2CKCFC_7th2Cross_Life2CrossStrait2EarthQuake262EarthQuake432FITNESS2HsinTien2iOS2KingdomHuang2Korea_Travel2KS93-3192MartialArts2marvel2MCU_Talk2media-chaos2medstudent2MINGDAO2Navy2NCCU07_SOCIO2NDMC-N572NHLUE-ILT2NKUTEE2NSwitch2NTUGIPO_PNSL2pts2SCU_Law101D2Soft_Job2swim2Theater2Therapist2THU_BA20002TKU_BF93C2TY_Research2visualband2Weyslii2Wrestle2WuLing46-3172XBOX2yoga2Yunlin2ADS1Atheism1BCC_Midnight1Beauty1Bingo1BoardGame1C_ChatBM1C_Question1CCSH_91_3151CCU-3DVG1chatskill1Christianity1CHSH_98_3011ck55th3141ck55th3191ck_17_3011cksh79th161cksh85th3011creditcard1CSMU-BS941CSMU-MED931DC1Disabled1DummyHistory1ELSWORD1Emulator1fastfood1FCU_Talk1FJU-EE-VLSI1FJU_JCS111GameDesign1GatoShoji1Geography1Ghost-Shell1graduate1Hiking1HK-movie1HSNU_10351Ikariam1ILSH_923121JP_Entertain1KHCHS-93-3091KingofPop1KOU1KS96-3051KS97-3161KS97-3171LawBasketbal1LHU1Lifeismoney1LifS_Service1LoveLive1LTU1MapleStory1medache1MenTalk1money1Nangang1NCCU_poetry1NCCU_SEED1NCHU-FS1001NCHU-FS981NCHU-FS991NCHU_CsHsnu1NCU_MATH861NCU_ME-94B1NDMC-M1031NDMC-M1081NDS1NetSecurity1NEW_ROC1NHU_LD921NHU_Talk1NightLife1Nintendo1NIU-ECE94b1NKFUST-CCE901NSYSU-PE1021NTPU-CSIE981NTPU-STAT951NTU-HTvolley1NTU_Service1NTUBST991NTUE-CS991NTUE-CUL1011NTUmed911NTUMRC1NTUST-EE-A971NTUST_Talk1NTUT_EE490A1NTUT_IPET4951Nurse1NUTN_MS991ObataTakeshi1Odoko-juku1outdoorgear1P_Management1PC_Shopping1petwork1PhD1PHI_Basketba1PhotoCritic1Physics1politics1PSY1PttLifeLaw1Railway1regimen1SFFamily1Shu-Lin1SocialTeam1StarTrek1Steam1studyteacher1STUST1SW_Job1TA_AN1Tea1Tennis1TFSHS69th3181Thailand1THU_PH901TKU-Strategy1TMU9711ToS1TSH97_YK1TTSH12th3091TTU-AFL1TTU-talk1UFO1Vancouver1Viator96Chia1Viator96Gang1Virgo1VISA1Wargaming1WashingtonDC1WindowsPhone1WuLing50-3041XiangSheng1Yup02-091<< 收起看板(326)

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[ CSMU-LS89 ]23 留言, 推噓總分: +13

作者: hipper - 發表於 2006/03/17 15:11(20年前)

4^F推Anvec:03/17 22:08

9^F推Anvec:你喜歡就好03/19 01:05

15^F推Anvec:從前從前在西邊有一座高山03/20 17:37

16^F→Anvec:山明水秀　鳥語花香03/20 17:37

18^F→Anvec:大家都喜歡去那邊爬山踏青交遊兼放牛03/20 17:39

19^F→Anvec:常常爬山爬的腰酸背痛　故稱斷背山03/20 17:39

20^F→Anvec:哇哩勒　有人搞破壞啦 (踢踢踢踢踢03/20 17:40

21^F推Anvec:在實驗室不好好做實驗　上什麼B 　這樣對得起你的兔子嗎?03/20 17:41

22^F→Anvec:快去跟你女朋友吃晚餐啦嗚~ (哭著跑開03/20 17:41

跑膠的問題

[ CSMU-LS89 ]14 留言, 推噓總分: +7

作者: goodman1 - 發表於 2006/03/17 11:19(20年前)

6^F推Anvec:有沒有可能是第三步驟extension的時間不夠?03/17 17:38

7^F→Anvec:有的人會把annealing跟 extension 合併03/17 17:39

8^F→Anvec:但是如果 template的length > 250bp 就會來不及聚合反應03/17 17:40

[狂賀] 哈哈，終於成功啦

[ CSMU-LS89 ]4 留言, 推噓總分: +2

作者: zzfly - 發表於 2006/03/15 02:19(20年前)

2^F→Anvec:恭喜啦 @_@03/15 08:33

Southern blot的問題

[ CSMU-LS89 ]6 留言, 推噓總分: +2

作者: goodman1 - 發表於 2006/03/14 16:06(20年前)

1^F推Anvec:可以吧可是fold change可能要5倍以上才可以看出差異03/14 20:59

2^F→Anvec:如果你是load cDNA的話用Real-time PCR比較快又敏感03/14 21:00

3^F→Anvec:real time PCR可以看出2倍的差異03/14 21:02

[閒聊]經濟學的邊際效用遞解法則

[ CSMU-LS89 ]5 留言, 推噓總分: +4

作者: hipper - 發表於 2006/03/14 14:49(20年前)

3^F推Anvec:是沒有喝到吐啦可是也不用特別記一天幾瓶吧 ~"~03/14 22:41

4^F→Anvec:你幹麻那麼注意我 (羞)03/14 22:42

[狂賀]白色情人節

[ CSMU-LS89 ]8 留言, 推噓總分: +5

作者: hipper - 發表於 2006/03/14 14:31(20年前)

7^F推Anvec:樓上已經收到禮物了... 斷背...03/15 08:31

Re: [活動] 台北小聚餐

[ CSMU-LS89 ]10 留言, 推噓總分: +5

作者: Anvec - 發表於 2006/03/12 17:19(20年前)

1^F→Anvec:忘了說啦這家店的份量很大超級大碗公03/13 11:35

2^F→Anvec:一碗約四百多塊份量足夠2~3人吃03/13 11:35

3^F→Anvec:所以麵可以兩三個人share 然後加上湯前菜跟甜點03/13 11:36

7^F推Anvec:我跟樓上上是清白的03/14 00:26

8^F→Anvec:給四樓的我去年有在柳川辦流水席呀 @_@03/14 00:27

10^F推Anvec:我改訂25位03/14 13:06

[徵求

[ CSMU-LS89 ]11 留言, 推噓總分: +6

作者: hipper - 發表於 2006/03/12 13:40(20年前)

2^F推Anvec:那個東西是多多益善不太可能有用不到的03/12 15:54

5^F→Anvec: 白痴...@@03/14 00:28

6^F→Anvec:歪了 XD03/14 00:28

8^F推Anvec: =====================以上為白癡名單==================03/14 16:16

10^F→Anvec:勾代03/14 22:42

Re: [活動] 台北小聚餐

[ CSMU-LS89 ]10 留言, 推噓總分: +8

作者: Anvec - 發表於 2006/03/11 00:40(20年前)

3^F推Anvec:古拉爵也可以單點啦 @.@a03/11 15:01

4^F推Anvec:Ya 14人胡曉棠排除萬難要跑來03/11 17:20

5^F推Anvec:Ya 又被我拐來一個了陳大雄03/11 17:25

7^F推Anvec:okok03/12 00:44

8^F→Anvec:陽明的人快跟我聯絡呀 >.< 我的通訊錄不見了03/12 00:44

9^F推Anvec:漏了一個守宮 o.Oa03/12 01:14

[情報]冷氣團報到12日起降溫　玉山、合歡山有機會降雪！

[ CSMU-LS89 ]4 留言, 推噓總分: +3

作者: hipper - 發表於 2006/03/10 18:57(20年前)

2^F→Anvec:齁你不懂啦等你長大了就知道了03/10 20:18

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