Re: [重要] 遺傳工程課本
Histidine 3個pKa: 1.8, 6.0, 9.2
求pH5.4時帶正1價的His佔有的比例
pH = 5.4 => [H+] = 10^(-5.4)
[His+][H+]
————— =10^-1.8 => [His+] = [His2+] * 10^3.6
[His2+]
[His0][H+]
————— =10^-6.0 => [His0] = [His+] * 10^(-0.6)
[His+]
[His-][H+]
————— =10^-9.2 => [His-] = [His0] * 10^(-3.8)
[His0]
=> [His0] = [His2+] * 10^3
[His-] = [His2+] * 10^(-0.8)
10^3.6
(His+)% = ————————————————— * 100 %
1 + 10^3.6 + 10^3 + 10^(-0.8)
= 79.908 % ≒ 80%
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