[求救] 人類紅血球的螢光染色
想要請教各位生醫底子比在下強n倍高手，關於紅血球的螢光染色用nile red是否恰當
小弟的染色步驟是先取3ul的血球溶到等滲透壓的蔗糖水溶液內加以輕微晃動分散，
接著將nile red染劑2ul加入，一樣輕微晃動5分鐘使其均勻混和，最後以離心置換蔗糖
水溶液的方式將多餘的染劑去除掉(清洗兩次)，再加入調整過導電度的等滲透壓buffer
可是問題來了，經過離心清洗過後紅血球變得相當難分散，在顯微鏡底下確認過都聚集
成一團一團的狀況，想請教的問題是有可能是nile red的影響導致抗凝血劑失效嗎，又
或是說抗凝血劑本身有問題?
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