[討論] SDS-PAGE running buffer調pH?
許多protocol跟各大廠說明書
都建議配置10X running buffer時不要調整pH
想請問各位家裡的會不會調整?
因為我之前跑2D是使用沒調整的來跑 解析度還不賴
而剛剛學妹配置的10X stock有使用HCl調整pH
很擔心會影響我2D-PAGE的解析度阿 T_T
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