[求救] cell lysis buffer成分的用處
Cells were lysed either in Triton cell lysis buffer (10mM Tris, pH 7.05,30 mM
NaPPi, 50mM NaCl, 1% Triton X-100, 2mM Na3VO4, 50mM NaF, 20mM
β-glycerophsphate and freshly added 0.5 mM PMSF, 0.5 μg/ml Leupeptin,
0.5 μg/ml pepstatin, 1μg/ml microcystin)
想知道誇號內成分在lysis buffer的用處
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