[方法] How to make Bgl II site?

看板Biotech作者 (馬車從天上掉下來￾ ￾N)時間17年前 (2007/07/04 05:37), 編輯推噓1(1推 0噓 0→)
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Please tolerate that I cannot type Chinese. I recently confronted a problem. Now I have a plamid without Bgl II site. However two Bgl II sites are required for linking to a vector as a commercial kit. How to make two Bgl II sites into my plasmid? Thanks -- ※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 141.211.156.210
推
07/04 08:09, , 1F
用 primer 合成 BglII 插進去, 或直接接 BamHI site
07/04 08:09, 1F
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文章代碼(AID): #16Yi6na0 (Biotech)
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文章代碼(AID): #16Yi6na0 (Biotech)