[討論] 普植實驗P19
~洋蔥根尖細胞前處理及染色方法~
root tip 2~3cm
|
|
2m< 8-hydroxyquinoline <-----centromere 清楚
| 4hr at 18~20度C chromosome 縮短
|
95% alcohol:glacial acetic acid(3:1)solution <-----chromosome 固定
| overnight
|
distilled water
| 3min(twice)
|
1N HCl <-----------除去purime使DNA露出
|60度C 8min aldehyde group 與leuco-
| basic fuchsin作用染色
water rinse
| 2~3min
|
leuco-basic fuchsin <------DNA染色
|1~2hr(in dark)
|
1% pectinase <------除去middle lamella 使細胞打開
|
|
45% acetic acid <------軟化細胞
|
|
exam.
這是有人用手機照下來~但無奈沒電腦
所以拜託我代PO的~
還是一樣.如果有錯誤.
還請大家幫忙訂正.
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