[建議] 又來PO文囉
Three-Dimensional Super-Resolution Imaging by Stochastic Optical
Reconstruction Microscopy
Summery:
Microscopy is processing today. Twenty years ago, the resolution of light
microscope was nearly 200 to 300nm. If you use those “super-resolution”
far-field microscopy techniques like stimulated emission depletion (STED)
and its related RESOLFT microscopy; stochastic optical reconstruction
microscopy (STORM), you will have the resolution about 20 to 50nm. But all
the resolutions only talk about 2D. There is a big barrier in 3D resolution.
The author’s group demonstrates 3D imaging with a spatial resolution that
is 10 times better than the diffraction limit in all three dimensions without
invoking sample or optical beam scanning. They exploit some reversible
fluorophores like Cy3, Cy2, Alexa 647. These fluorophores can be reversibly
cycled between a fluorescent and a dark state by light of different
wavelength This reversible switching behavior provided an internal control to
measure the localization accuracy.
The break through in the paper is they demonstrated a 3D STROM by using
optical astigmatism to determine both axial and lateral position of
individual fluorophores with nanometer accuracy.
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04/16 23:39, , 1F
04/16 23:39, 1F