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看板NTHU_FC作者 (期待)時間18年前 (2008/03/18 14:30), 編輯推噓0(000)
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A new p38 MAP kinase-regulated transcriptional coactivator that stimulates p53-dependent apoptosis Abstract: When cells feel the environmental stresses such as UV or cisplatin treatment, they will go from cell-cycle arrest to apoptosis through specific signaling pathways. Among them the p38 mitogen-activated pretein kinase (MAPK) signaling pathway is one of the most important cell-fate decisions. P38α is the most widely studied member of the p38 MAPKs in higher eukaryotes.It’s always activated by the MAPK kinases MKK6,MKK3 and MKK4 by phosphorylat- ion. The ability of p38α may function as a tumor suppressor, which can positively regulate several tumor suppressor pathways and attenuate oncogenic signals. The role of p38α is to regulate the tumor suppressor protein p53 through phosphorylati- on of p53. When p53 has been activated, it can lead to reversible cell-cycle arrest, an irreversible senescence-like state or apoptosis. When the author’s team have identified a protein of 18kDa, which named p18Hamlet specifically interact with human p38α. They found that p18Hamlet protein are regulated by p38 MAPK. And p38 MAPK activation by UV treatment also correlated with Thr phosphorylation of p18Hamlet. Because this phosphorylation will be significa- ntly reduced when the cells were pretreated with the p38 MAPK inhibitor SB203580. The protein so-called p18Hamlet will accumulate when a cell experiencing DNA damaging. And then the phenomenon will activate p53-dependent gene promoters such as NOXA and PUMA due to the C-terminal zinc-finger HIT domain of p18Hamlet. At last it causes cell-cycle arrest or apoptpsis. Therefore, the author finds a new cell-fate regulator named p18Hamlet , which contributes to the implementation of p53-regulated cellular responses. -- ※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 140.114.96.155
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