[求救] pfu DNA polymerase廠牌推薦

看板Biotech作者 (VincentYan)時間7年前 (2017/04/17 10:06), 編輯推噓11(11推 0噓 7→)
留言18則, 12人參與, 最新討論串1/1
各位大大好， 小弟目前以Thermo牌的pfu進行site-directed mutagenesis， 實驗未能做出來，pfu卻在幾週前用完了 但殊不知Thermo日前已經停產pfu了... (順便一提, 它的T4 ligase也漲的超級誇張) 請問各位版友，有推薦pfu的廠牌嗎? 感謝大家!! -- Sent from my Windows -- ※ 發信站: 批踢踢實業坊(ptt.cc), 來自: 140.112.4.190 ※ 文章網址: https://www.ptt.cc/bbs/Biotech/M.1492394812.A.F33.html
推
04/17 10:41, , 1F
KOD或Phusion
04/17 10:41, 1F
推
04/17 12:18, , 2F
PfuUltra II Fusion HS (Agilent)
04/17 12:18, 2F
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04/17 12:18, , 3F
Bioline
04/17 12:18, 3F
→
04/17 12:18, , 4F
BIOLINE 捷昇
04/17 12:18, 4F
推
04/17 17:42, , 5F
KOD+1
04/17 17:42, 5F
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04/17 18:45, , 6F
Phusion、Q5
04/17 18:45, 6F
推
04/17 20:12, , 7F
phusion跟q5太強了 做sdm常常做不好
04/17 20:12, 7F
推
04/17 23:37, , 8F
我用kod很好
04/17 23:37, 8F
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04/18 02:40, , 9F
Phusion做cloning不錯, SDM我是用KOD
04/18 02:40, 9F
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04/18 02:41, , 10F
T4 ligase都用NEB的 沒比過價格
04/18 02:41, 10F
推
04/18 08:04, , 11F
但Q5本身就有被搭配Kit了，goo.gl/EoSXPY，我覺得是OK的耶
04/18 08:04, 11F
→
04/18 08:04, , 12F
XD
04/18 08:04, 12F
推
04/18 08:48, , 13F
PerfectRead Plus
04/18 08:48, 13F
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04/18 09:46, , 14F
transgen
04/18 09:46, 14F
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04/18 09:47, , 15F
transgen fastpfu
04/18 09:47, 15F
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04/18 13:16, , 16F
感謝大家熱情回應，目前有問到其他家代理商的Thermo p
04/18 13:16, 16F
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04/18 13:16, , 17F
fu庫存，還是先用它(老師指示)
04/18 13:16, 17F
推
05/14 18:52, , 18F
thermo f-530還不錯用...
05/14 18:52, 18F
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