[求救] TTC stain
最近我要做小鼠心臟缺血的實驗，
需要用TTC stain(Triphenyltetrazolium chloride)區隔出缺血壞死的區域。
我看paper查了Protocol
The hearts were sliced transversely from the apex to base into 5
sections. Then, they were incubated for 30 min in 2% TTC
(Sigma) solution at 37uC and fixed in 4% paraformaldehyde
overnight. The infarcted myocardium (negative for TTC staining,
white) and the non-ischemic zones (stained brick red) were divided
and weighed.
大致是說將心臟做五連切切片(還是分成五等份?)
接著泡在2%的TTC solution中在37度C 30分鐘，
我疑惑的是，這方法是否適用於石蠟切片?如果適用，需要脫蠟嗎@@?
我是有查到凍切拿來做TTC stain的，但是凍切對我有點小苦手。
因此上來求教是否有前輩做過能指點迷津呢?
感激不盡，謝謝!!
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