[求救] 如何從全血中得到比例較多的血清(人)
發問時請注意，若是要問實驗相關的問題，請將實驗的步驟、所用的條件大致列出
以方便板友幫您追蹤問題
----請將上列注意事項以 ctrl + k 刪除後開始編輯文章----
大家好,這是我第一次進入本版
好像出現一線曙光的感覺
由於我本身是機械背景,現在正在做與血液相關的實驗,因為計畫要用人血,
而我只好抽自己的血作實驗了(一次實驗至少需要16CC的血清)
剛剛很認真的爬文,發現要從人類的全血中離心出較高比例的血清(約莫6成)
只有一位板友分享過一本書上的內容 如下
1. After collection, blood should be allowed to clot for 30-60 min at 37C
2. The clot should then be separated from the sides of the collection vessel
(ringing)using a Pasteur pipette, straightened paper clip, or similar
instrument. Place the colt at 4C overnight allow it to contract.
3. The serum should then be removed from the clot and any remaining insoluble
material removed by centrifugation at 10,000g for 10 min at 4C
After separation, serum can be stored for many year at -20C or below.
但礙於經費問題 實驗室只有1300G的經濟型離心機
想請教各位生科前輩
有無其他離心方法可以生出比例較高的血清
或是有其他管道可以取得人體血清
<(_ _)> 謝謝各位前輩解惑
--
※ 發信站: 批踢踢實業坊(ptt.cc)
◆ From: 140.112.38.128
推
02/02 20:52, , 1F
02/02 20:52, 1F
→
02/02 21:15, , 2F
02/02 21:15, 2F
推
02/02 21:17, , 3F
02/02 21:17, 3F
→
02/02 21:19, , 4F
02/02 21:19, 4F
推
02/02 21:26, , 5F
02/02 21:26, 5F
→
02/02 21:27, , 6F
02/02 21:27, 6F
→
02/02 21:28, , 7F
02/02 21:28, 7F
→
02/02 21:29, , 8F
02/02 21:29, 8F
→
02/02 21:30, , 9F
02/02 21:30, 9F
推
02/02 21:33, , 10F
02/02 21:33, 10F
→
02/02 21:36, , 11F
02/02 21:36, 11F
推
02/02 21:39, , 12F
02/02 21:39, 12F
→
02/02 21:45, , 13F
02/02 21:45, 13F
→
02/02 21:47, , 14F
02/02 21:47, 14F
→
02/02 21:54, , 15F
02/02 21:54, 15F
推
02/02 23:27, , 16F
02/02 23:27, 16F
→
02/03 00:34, , 17F
02/03 00:34, 17F
→
02/03 00:35, , 18F
02/03 00:35, 18F
推
02/03 09:48, , 19F
02/03 09:48, 19F
推
02/07 07:22, , 20F
02/07 07:22, 20F
→
02/07 07:23, , 21F
02/07 07:23, 21F
→
02/07 07:24, , 22F
02/07 07:24, 22F
→
02/15 00:23, , 23F
02/15 00:23, 23F