[求救]為何都測蛋白質濃度了,跑出的western band
發問時請注意，若是要問實驗相關的問題，請將實驗的步驟、所用的條件大致列出
實驗為測定核質分離的蛋白質濃度(細胞實驗),但每次測完蛋白質濃度,western跑完膠後,理當不同時間點未加藥之控制組的核內蛋白表現量應該一致,可每批細胞跑出來的表現量卻是不一致,internal control則和目標蛋白表現量的趨勢是一樣的,其也會受影響,
但沒有細胞質蛋白的汙染.不知道未什摸會這樣
以方便板友幫您追蹤問題
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