[求救] 跪求E.coli strain: BL21-codon plus(D …
我是交大的學生
最近在表現一個分子量100KD多的蛋白
我選用pET30b做vector 表現量卻不高 (IN Novablue(DE3) & BL21 (DE3))
由於表現的基因GC％很高 因此懷疑可能因此造成表現量較低
希望版上哪位先進有
E.coli strain: BL21-codon plus(DE3)-RP
或是
E.coli strain: BL21-codon plus(DE3)-RIPL
或是
Rosetta 系列的菌（目的是想補充稀有密碼子,目前最想要這隻...）都可以
的菌株....可以向您要...我可以買飲料或是中晚餐交換QQ
如果再比較遠的地方我可以付郵寄費用....謝謝,....
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※ 發信站: 批踢踢實業坊(ptt.cc)
◆ From: 140.113.173.247
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※ 編輯: mournermind 來自: 140.113.173.247 (08/15 01:16)
※ 編輯: mournermind 來自: 140.113.173.247 (08/15 01:24)
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