[期刊] Design and Application of a shRNA-Based Gene Replacement

看板Biotech作者 (AJ)時間15年前 (2010/08/21 13:05), 編輯推噓1(1012)
留言13則, 5人參與, 最新討論串1/1
1. 這篇文章大概描述(中文30字).... Using virus, expression of the endogenous Anti-Silencing Function 1a (ASF1a) histone chaperone has been efficiently replaced in primary human cells, by an ectopically expressed epitope-tagged version. Moreover, the virus is designed so that other shRNA and shRNA-resistant cDNA cassettes can easily be substituted, making the approach readily applicable to other protein targets. [期刊名稱 與 標題卷號年份等資訊] 1.Methods in Molecular Biology, 2007, Volume 408, III, 211-221, DOI: 10.1007/978-1-59745-547-3_12 [期刊網址/ 太長的話 請到http://tinyurl.com縮網址] 1.http://tinyurl.com/2ekkjrx [我的e-mail] m9613030@ems.ndhu.edu.tw 跪求各位先進~感恩~~ -- ※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 140.120.208.42

08/21 16:32, , 1F
好沒誠意呀,簡介直接剪貼 ...
08/21 16:32, 1F

08/21 17:55, , 2F
SORRY~由於剛進博班新實驗室 領域一竅不通 老闆突然要我
08/21 17:55, 2F

08/21 17:55, , 3F
幫他找這篇...一時也不知道怎麼說明~
08/21 17:55, 3F

08/21 20:07, , 4F
基本上就我的瞭解 這期刊比較像一本書 不會有單獨的pdf
08/21 20:07, 4F

08/21 20:12, , 5F
如果想拿到 其實你可以拿去 google 看看運氣和耐心了..
08/21 20:12, 5F

08/21 20:13, , 6F
不然就去 nature protocl 找篇類似的給老師吧...
08/21 20:13, 6F

08/21 21:02, , 7F
好的 感謝樓上O大~!!
08/21 21:02, 7F

08/21 22:22, , 8F
Methods in molecular biology精裝版各大學館藏較多。
08/21 22:22, 8F

08/21 22:45, , 9F
這篇文章是用針對UTR的序列來設計shRNA另外再加入CDS的
08/21 22:45, 9F

08/21 22:46, , 10F
OVEREXPRESSION在同一個質體內
08/21 22:46, 10F

08/21 22:47, , 11F
下不為例喔,因為有違板規,希望你看完後,可以改一改
08/21 22:47, 11F

08/21 22:47, , 12F
done~
08/21 22:47, 12F

08/21 22:52, , 13F
ORZ 感謝ran大!!!!
08/21 22:52, 13F
文章代碼(AID): #1CRrwqkW (Biotech)