[求救] primary cell汙染
求救一下,最近養primary cell進入第四個月,
實驗室的primary cell大概長2星期左右就可以做實驗.
前兩個月好好的,但在前陣子進了一個新人後,
開始出現鏈球菌和霉漿菌的混合汙染,
接著就不得安寧.....隔幾週就汙染一次,
primary cell不能丟,只能加抗生素盡量救,
但這次的鏈球菌加了gentamycin和pen-strep下去,
完全抑制不了汙染,好像已經有抗藥性了,
都會在汙染後的第2~3天開始侵入細胞....
看了前面的文章,發現不點酒精燈似乎比較好,
打算試試看,
但我們已經把incubator滅菌.戴口罩手套.開UV等,
能做的都做了,還是出現汙染,
請問版上的各位,還有什麼建議和經驗可以幫助我戰勝鏈球菌....
thanks!!
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