[求救] 如何選擇細胞株
我第一次要買細胞株
老師叫我去ATCC上看 我要買的是CHO CELL 但是一進去有37種的CHO
真的搞得我霧煞煞
裡面有些細胞株提到
This line is Neomycin resistant.
The cell line was derived by co-transfecting the CHO cell line DXB11 using
Lipofection with heavy and light chain expression plasmids constructed from
the antibody produced by the 23F2G cell line (ATCC -HB-11081).
The plasmids contain both cytomegalovirus (CMV) and SV40 viral sequences.
The 23F2G antibody is reactive with the beta chain (CD18) of human leukocyte
integrins.
由於我即將要Transfect的plasmid 沒有細胞可辨識的selective gene
老師說
我可以考慮試試看cotransfection for stable cell line
以下是我的解讀 請大家指正
是指說這些細胞株本身就有可以抑制neomycin的功能
我若將我自己的plasmid用共轉染的方式放入cell中 可以做stable transfection
而不是只能做transient transfection
是這個意思嗎?
請大家幫幫忙
我該如何選擇細胞株呢?
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