[求救] 0.5% triton insoluble protein是不是很少?!
最近用polarized MDCK cell去作triton X-100 solubulity assay
看一些cytoskeleton-associated protein
用0.5% triton處理過後的細胞,絕大部分的protein都已經溶出(triton soluble)
剩下的pellet部份就是triton insoluble fraction
超難溶,用1% SDS去溶而且去測濃度(有時會sonicate/加熱很久)
濃度都很低
而且跑出來的band很像毛毛蟲,或常因protein量太少而壓不到band
很困擾....
不知有無辦法可改善insoluble fraction 的 western quality?!
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