[方法] DCFH-DA assay in 293T

看板Biotech作者WHATISGACKT時間15年前 (2009/01/06 18:12)推噓1(1推 0噓 0→)

留言1則, 1人參與討論串1/1

因為最近某個實驗要測ROS 可是reagent其實放了一陣子想先test一下 DCFH-DA是否還可以使用? here is the protocol 1. 293T without serum, 37 degree C, for 15 mins 2. add DCFH-DA 50 microM, 37 degree C, for 15 mins 3. trypsin digestion 4. resuspend in PBS 5. flowcytometry analysis i was wondering if there is any step could be modified ? thanks -- ※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 140.112.129.10 ※ 編輯: WHATISGACKT 來自: 140.112.129.10 (01/06 18:13)

推

paua

01/07 11:59, , 1^F

01/07 11:59, 1^F

‣ 返回看板[ Biotech ] BIO

‣ 更多 WHATISGACKT 的文章

文章代碼(AID): #19OowG2D (Biotech)