[求救] 請問測PKC-zeta assay

看板Biotech作者 (coolmanred)時間17年前 (2008/11/11 00:50), 編輯推噓1(1推 0噓 1→)
留言2則, 2人參與, 最新討論串1/1
請問在測PKC activity時 利用免沉澱法 加入 MBP(myelin basic protein)的目的是什麼? The immunoprecipitates were incubated with 2 μg MBP, 10 μCi [γ-32P]–ATP, 100 μM ATP for 30 min at 30°C. The proteins were separated by SDS-PAGE, and radiolabeled MBP was detected by autoradiography. 謝謝 -- ※ 發信站: 批踢踢實業坊(ptt.cc) ◆ From: 218.166.213.193
推
11/11 01:20, , 1F
MBP是PKC的substrate,藉由label的isotope可以測PKCactivity
11/11 01:20, 1F
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11/11 21:47, , 2F
謝謝囉
11/11 21:47, 2F
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