[求救] 幾提生化問題
1.(a) In gel filtration, larger proteins run faster or slower? Why?
我知道大的跑比較快 但不知道為什麼<囧
(b) In SDS-PAGE, larger proteins run faster or slower? Why?
我知道大的跑比較慢 但不知道為什麼<囧
2.(1) Can an antibody and its antigen be co-crystallized for X-ray
determination to study the interaction between this antibody
and its antigen? Why?
(2) Can we apply the same idea to study the interaction between
an enzyme and its allosteric effector or substrate individually
via co-crystallization? Why?
3.(a) Why do we add PITC in the analyses of amino acid composition
and sequencing?
(b) Why do we add 6 M HCl in the analysis of amino acid composition
but not in the analysis of amino acid sequencing?
(c) The existence of disulfide bonds in a protein blocks the Edman
reaction. How to solve this problem during amino acid sequencing?
請高手指點迷津 感激不盡:)
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※ 編輯: steffanie 來自: 61.58.155.102 (10/30 22:17)