想請問段落翻譯
不好意思請問大家
這段的resuspend到底是在哪個地方呢
我查了原來灌洗的方法還是跟老師提供的paper敘述方法一樣
麻煩大大提供意見
感恩
以下
Nasal Lavage
Nasal lavage was performed as described earlier with some modifications (12).
Briefly, 4.5 ml of prewarmed Hanks' balanced salt solution (HBSS) (37° C)
was instilled through a heat-softened catheter into the nare. The subject
held his or her chin down toward the chest and held the catheter in place by
pinching the nares closed. The cartilaginous bridge of the nose was vibrated
by a pediatric precursor while the fluid was refluxed three times. The lavage
fluid was recovered and the procedure repeated in the opposite nare. The
lavage fluid was placed immediately on ice until processing. Thereafter the
lavage fluid sample was centrifuged, the cells were resuspended in 2 ml of
supernatant of the lavage fluid, and the rest of the cell-free supernatant
was aliquoted and frozen.
到底是
1.抽到剩下2cc resuspened
2.抽上清液2cc 下面沉澱物拿去2cc resuspend
3.抽上清液兩cc resuspend(....)
因為有點不太了解字面上嚜意思
希望有德的大大可以解惑
感恩
A volume of 100 μl of resuspended cell suspension
was used for cytospin; the remaining cell suspension was incubated for 20 h
at 37° C, and centrifuged. The cells were frozen for Western blot analysis
of iNOS. Nitrite production was measured in 100 μl of the supernatant, with
the remainder being collected and frozen for later cytokine analysis.
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