[討論] 從皮膚細胞變成幹細胞
今天一條很大的新聞
日本與美國兩個研究團隊同時在今天發表一項研究成果
表示只要在一般人的皮膚細胞中入四個基因後
即可將該皮膚細胞轉為幹細胞
不但外觀型態與幹細胞無異
此一轉型後的幹細胞也能分化成不同的細胞
此項技術將會為幹細胞研究與很多疾病治療的研究帶來重大的突破
新聞如下
http://sciencenow.sciencemag.org/cgi/content/full/2007/1120/1
Researchers Turn Skin Cells Into Stem Cells
By Gretchen Vogel
ScienceNOW Daily News
20 November 2007
Scientists have managed to reprogram human skin cells directly into cells
that look and act like embryonic stem (ES) cells. The technique makes it
possible to generate patient-specific stem cells to study or treat disease
without using embryos or oocytes--and therefore could bypass the ethical
debates that have plagued the field. "This is like an earthquake for both the
science and politics of stem cell research," says Jesse Reynolds, policy
analyst for the Center for Genetics and Society in Oakland, California.
The work builds on a study published last year by Shinya Yamanaka of Kyoto
University in Japan, which showed that mouse tail cells could be transformed
into ES-like cells by inserting four genes (ScienceNOW, 3 July 2006). Those
genes are normally switched off after embryonic cells differentiate into the
various cell types. In June this year, Yamanaka and another group reported
that the cells were truly pluripotent, meaning that they had the potential to
grow into any tissue in the body (ScienceNOW, 6 June).
Now the race to repeat the feat in human cells has ended in a tie: Two groups
report today that they have reprogrammed human skin cells into so-called
induced pluripotent cells (iPCs). In a paper published online in Cell,
Yamanaka and his colleagues show that their mouse technique works with human
cells as well. And in a paper published online in Science, James Thomson of
the University of Wisconsin, Madison, and his colleagues report success in
reprogramming human cells, again by inserting just four genes, two of which
are different from those Yamanaka uses.
In the new work, Yamanaka and his colleagues used a retrovirus to ferry into
adult cells the same four genes they had previously used to reprogram mouse
cells: OCT3/4, SOX2, KLF4, and c-MYC. They reprogrammed cells taken from the
facial skin of a 36-year-old woman and from connective tissue from a
69-year-old man. Roughly one iPC cell line was produced for every 5000 cells
the researchers treated using the technique, an efficiency that enabled them
to produce several cell lines from each experiment.
Thomson's team started from scratch, identifying its own list of 14 candidate
reprogramming genes. Like Yamanaka's group, the team used a systematic
process of elimination to identify four factors: OCT3 and SOX2, as Yamanaka
used, and two different genes, NANOG and LIN28. The group reprogrammed cells
from fetal skin and from the foreskin of a newborn boy. The researchers were
able to transform about one in 10,000 cells, less than Yamanaka's technique
achieved, Thomson says, but still enough to create several cell lines from a
single experiment.
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